Construction and anti-tumor effects of recombinant fowlpox virus expressing Newcastle disease virus hemagglutinin-neuramidinase gene

Hemagglutinin-neuramidinase (HN), a Newcastle disease virus-derived protein, not only mediates receptor recognition but also possesses neuraminidase (NA) activity, the ability to cleave a component of those receptors, N-acetylneuraminic acid (NAcneu, sialic acid). It is known that this protein in mammalian species, including human beings, has interesting anti-neoplastic as well as immune stimulating properties. To explore the use of the HN gene in cancer gene therapy, we constructed a recombi-nant fowlpox virus expressing the HN protein (vFV-HN) and compared the anti-tumor activity of the recombinant virus with that of wild-type fowlpox virus (FPV) in vivo and in vitro. Here we found that although B16 cells were somewhat resistant to the basal cytotoxic effect of wild-type fowlpox virus, infection with vFV-HN caused a pronounced cytotoxic effect and, the survival of tumor-bearing mice immunized with vFV-HN was significantly increased compared with the survival of mice immunized with the FPV alone. Furthermore, the immunization of mice with vFV-HN elicited a B16 tumor-specific cytotoxic T lymphocyte (CTL) response and clonal expansion of both CD4 and CD8 T cell populations in vivo. In addition, T cells from lymph nodes of mice vaccinated with vFV-HN secreted high levels of the Th1 cytokine IL-2 and IFN-γ, indicating that the regression of tumor cells is related to a Th1-type dominant immune response. These results demonstrate that vaccination with vFV-HN may be a potential strategy for cancer gene therapy.     

A new frequency scale of Chinese whispered speech in the application of speaker identification

In this paper, the frequency characteristics of Chinese whispered speech were investigated by a filter bank analysis. It was shown that the first and the third formants were more important than the other formants in the speaker identification of Chinese whispered speech. The experiment showed that the 800?1200 Hz and 2800?3200 Hz ranges were the most significant frequency ranges in discriminating the speaker. Based on this result, a new feature scale named whisper sensitive scale (WSS) was proposed to replace the common scale, Mel scale, and to extract the cepstral coefficient from whispered speech signal. Furthermore, a speaker identification system in whispered speech was presented based on the modified Hidden Markov Models integrating advantages of WSCC (the whisper sensitive cepstral coefficient) and LPCC. And the new system performed better in solving the problem of speaker identification of Chinese whispered speech than the traditional method.     

Na_3AlF_6-AlF_3-Al_2O_3-CaF_2-LiF-NaCl系电解质密度的研究

研究了电解质中加入氯化钠,并用氯化钠取代部分氟化锂的电解质体系的密度.采用电子天平与计算机串口相结合的测量技术,根据阿基米德定律确定电解质体系的密度,误差小于1%.利用该方法,通过四因素四水平正交实验研究了Na3AlF6-AlF3(10.87%~16.67%)-Al2O3(3%)-CaF2(4%)-LiF(0~6%)-NaCl(0~8%)体系电解质密度,并且根据测量结果建立了该体系的密度数学模型:tρ(g.cm-3)=2.897 1-0.003 07AlF3(w/%)+0.003 041LiF(w/%)-0.009 32NaCl(w/%)-0.000 85t(℃).分析了不同分子比、氟化锂添加...     

模糊粗糙集的不确定性度量

利用知识的信息熵和粗糙性给出了模糊粗糙集的不确定性度量的方法,讨论了度量指标的相关性质.实例表明,文中给出的度量对研究模糊粗糙集的不确定性具有指导作用.     

一次天津地区暴雨过程的中小尺度分析

用NCEP再分析资料、雷达资料以及地面自动站每小时资料对2005年6月28-29日发生在天津地区的局地暴雨天气进行诊断分析,研究结果表明:高低空急流耦合是暴雨发生发展的有利背景,由天津市西南方向移来的中尺度系统与市区地面中尺度辐合线共同作用是产生这次局地暴雨天气的直接触发机制。天津市区的局地条件在这次局地降水的过程中起到了相当重要的作用。     

国家农业科技园区技术对接机制的运行模式

技术对接功能是农业科技园区的核心功能之一,国家农业科技园区技术对接机制的运行模式是技术对接效果的决定因素。对国家农业科技园区技术对接机制的涵义及运行模式进行分析,归纳了四种国家农业科技园区技术对接机制运行的模式。     

《河北理工学院学报》被博硕论文引用的统计分析

《河北理工学院学报》有比较高的学术影响力,被2004年、2005年67所研究生学位授予单位的191篇博硕论文引用;博硕论文引用《学报》论文有鲜明的学科专业属性,相同、相近的学科专业之间互引度更高;《学报》被引论文的共同特点是理论性、实践性、创新性和综合性强。     

容栅数显卡尺同步显示软件设计

介绍了容栅式数显卡尺同步显示装置的硬件、软件设计.该同步显示装置是把数显卡尺RS232接口输出信号送入以8051单片机为基础的信号处理电路,配以数据接收、数据处理、数据显示等软件,实现测量信号远距离传输,该装置在机床标尺等数显装置中广泛应用.     

聚吡咯甲烯类聚合反应的动力学研究

采用原位紫外分光光度法研究了吡咯与苯甲醛，对二甲氨苯甲醛，香草醛及对硝基苯甲醛反应生成聚吡咯甲烯类聚合物过程中的合成反应机理。研究表明，聚吡咯甲烯的合成反应为一零级反应，其反应速率与催化剂的酸度有关，而与吡咯和醛的浓度无关，苯环上吸电子基团的存在有利于聚合反应的进行，较大空间位阻基团的存在则使聚合反应的速率下降。     

复合离子聚合物调剖剂深部调剖机理研究

对复合离子聚合物调剖剂的组成和化学反应原理进行了研究 ,根据大庆油田目前所用的配方 ,通过室内实验得到了相对分子质量不同的多种配方调剖剂 ,并对其成胶特点、在岩心中物理堵塞作用的大小、以及用量对调剖效果的影响进行了分析。研究结果表明 ,复合离子聚合物调剖剂的交联时间可以控制 ,成胶时间从 2天到十几天不等。成胶后强度大 ,粘度大于 2 0Pa·s且有效期长 ,在实验观察 9个月的时间里 ,冻胶的粘度没有明显降低。另外 ,复合离子聚合物调剖剂对地层的堵塞作用大 ,地层条件下可使渗透率降低 6 0 %以上。该调剖剂已在大庆油田进行了矿场试验 ,并取得了良好的调剖效果